Primary structure of p19 species of avian sarcoma and leukemia viruses.
نویسندگان
چکیده
The internal structural proteins of avian sarcoma and leukemia viruses are derived from a precursor polypeptide that is the product of the viral gag gene. The N-terminal domain of the precursor gives rise to p19, a protein that interacts with the lipid envelope of the virus and that may also interact with viral RNA. The C terminus of p19 from the Prague C strain of Rous sarcoma virus was previously assigned to a tyrosine residue 175 amino acids from the N terminus. We have used metabolic labeling and carboxypeptidase digestion to show that the C terminus of p19 is actually tyrosine 155. This implies the existence of a sixth gag protein 22 amino acids in length and located between p19 and p10 on the gag precursor. The p19 species of some recombinant avian sarcoma viruses and of the defective endogenous virus derived from the ev-1 locus migrate on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as if they were about 4,000 daltons smaller than p19. We have elucidated the structure of these forms, called p19 beta, by analysis of the proteins and determination of the DNA sequence of the p19 region of the gag gene from ev-1 and ev-2. Esterification of carboxyl groups completely suppressed the differences in migration of p19 and p19 beta. Peptide mapping showed the altered mobility to be determined by sequences in the C-terminal cyanogen bromide fragment of the proteins. We conclude from the DNA sequence that a single glutamate-lysine alteration is responsible for the altered electrophoretic mobility.
منابع مشابه
Genome structure of avian sarcoma virus Y73 and unique sequence coding for polyprotein p90.
The genome structure of a newly isolated sarcoma virus, Y73, was studied. Y73 is a defective, potent sarcomagenic virus and contains 4.8-kilobase (kb) RNA as its genome; in contrast, helper virus associated with Y73 had 8.5-kb RNA, similar to other avian leukemia viruses. Fingerprinting analysis these RNAs demonstrated that the 4.8-kb RNA contains a specific RNA sequence of 2.5 kb, which repres...
متن کاملCorrelation of RNA binding affinity of avian oncornavirus p19 proteins with the extent of processing of virus genome RNA in cells.
We purified the p19 proteins from the Prague C strain of Rous sarcoma virus, avian myeloblastosis virus, B77 sarcoma virus, myeloblastosis-associated virus-2(0), and PR-E 95-C virus and measured their binding affinities for 60S viral RNA by the nitrocellulose filter binding technique. The apparent association constants of the p19 proteins from Rous sarcoma virus Prague C, avian myeloblastosis v...
متن کاملTUMOR VIRUS RNAs AND TUMOR VIRUS GENES
The RNAs of several classes of avian and murine RNA tumor viruses were compared. The 60-70s RNA complex of cloned nondefective avian sarcomaviruses contains only 30-40s RNA species of class a, which are larger than the 30-40s RNA species of class b found in avian transformation-defective or leukosis viruses. The RNA of a recombinant avian sarcoma virus, carrying a host range marker of a leukosi...
متن کاملApparent DNA-binding protein specific for cells transformed by avian acute leukemia viruses.
An apparent DNA-binding protein is described that is specific for cells transformed by avian acute leukemia viruses. This protein cannot be demonstrated in cells transformed by any of the avian sarcoma viruses or in cells infected with nontransforming avian retroviruses. The protein also is not detectable in noninfected quail or chicken embryo primary cultures or in noninfected chicken hematopo...
متن کاملStructure and specific sequences of avian erythroblastosis virus RNA: evidence for multiple classes of transforming genes among avian tumor viruses.
Two major RNA species were found in several clonal isolates of avian erythroblastosis virus (AEV) and avian erythroblastosis-associated helper virus (AEAV) complexes: one of 8.7 kilobases (kb), the other of 5.5 kb. The 5.5-kb species was identified as AEV RNA because (i) it was absent from non-transforming AEAV isolated from the same virus complex, (ii) it was present in complexes of AEV and di...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of virology
دوره 56 1 شماره
صفحات -
تاریخ انتشار 1985